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pde activity assay kit  (Abcam)


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    Abcam pde activity assay kit
    Pde Activity Assay Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pde+activity+assay+kit/Ubiquitylation+Assay+Kit/pm33977557-89-24-23
    Average 99 stars, based on 57 article reviews
    pde activity assay kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    In Vitro:

    Article Title: Design, synthesis, and pharmacological characterization of some 2-substituted-3-phenyl-quinazolin-4(3H)-one derivatives as phosphodiesterase inhibitors.
    Article Snippet: Correspondence Riham F. George, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.. Email: rihamfgeorge@gmail.com Abstract Some 3‐phenyl‐quinazolin‐4(3H)‐one‐2‐thioethers (3a–e, 5a,b, 7a–e, 9a–d, 10a–d, and 12) along with 2‐aminoquinazoline derivatives 13a–c were prepared and screened for their in vitro phosphodiesterase (PDE) inhibitory activity.. Some compounds such as 7d,e, 9a,b,d, 10a,d, and 13b exhibited promising activity as compared with the non‐selective PDE inhibitor IBMX.

    Activity Assay:

    Article Title: Design, synthesis, and pharmacological characterization of some 2-substituted-3-phenyl-quinazolin-4(3H)-one derivatives as phosphodiesterase inhibitors.
    Article Snippet: Correspondence Riham F. George, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.. Email: rihamfgeorge@gmail.com Abstract Some 3‐phenyl‐quinazolin‐4(3H)‐one‐2‐thioethers (3a–e, 5a,b, 7a–e, 9a–d, 10a–d, and 12) along with 2‐aminoquinazoline derivatives 13a–c were prepared and screened for their in vitro phosphodiesterase (PDE) inhibitory activity.. Some compounds such as 7d,e, 9a,b,d, 10a,d, and 13b exhibited promising activity as compared with the non‐selective PDE inhibitor IBMX.

    Synthesized:

    Article Title: Design, synthesis, and pharmacological characterization of some 2-substituted-3-phenyl-quinazolin-4(3H)-one derivatives as phosphodiesterase inhibitors.
    Article Snippet: Correspondence Riham F. George, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt.. Email: rihamfgeorge@gmail.com Abstract Some 3‐phenyl‐quinazolin‐4(3H)‐one‐2‐thioethers (3a–e, 5a,b, 7a–e, 9a–d, 10a–d, and 12) along with 2‐aminoquinazoline derivatives 13a–c were prepared and screened for their in vitro phosphodiesterase (PDE) inhibitory activity.. Some compounds such as 7d,e, 9a,b,d, 10a,d, and 13b exhibited promising activity as compared with the non‐selective PDE inhibitor IBMX.



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    Image Search Results


    The loss of G i/o regulation by RGS2 and 5 augments uterine artery myogenic tone by downregulating cAMP generation and upregulating PDE activity. ( A ) Forskolin-stimulated cAMP generated in uterine arteries from wild type (WT), Rgs2 knockout ( Rgs2 −/− ), Rgs5 knockout ( Rgs5 −/− ), and Rgs2/5 double knockout ( Rgs2/5 dbKO) mice. Freshly isolated uterine arteries were incubated with forskolin (50 μ m ) for 5 min in the presence of 50 μ m IBMX. ( B and C ) Phosphodiesterase (PDE) activity in uterine arteries from WT, Rgs2 −/− , Rgs5 −/− , and Rgs2/5 dbKO mice. Freshly isolated uterine arteries were incubated with vehicle (0.01% DMSO) or PTX (750 ng m −1 ) for 2 h before processing for the PDE activity assay. Data points indicate assays with uterine arteries from individual mice. Values are mean ± SEM. *, ** P < .05, .01 versus WT control (con); # P < .05, Rgs2 −/− con versus Rgs2 −/− + PTX; and ns—not significant.

    Journal: Function

    Article Title: Phosphodiesterases Mediate the Augmentation of Myogenic Constriction by Inhibitory G Protein Signaling and is Negatively Modulated by the Dual Action of RGS2 and 5

    doi: 10.1093/function/zqae003

    Figure Lengend Snippet: The loss of G i/o regulation by RGS2 and 5 augments uterine artery myogenic tone by downregulating cAMP generation and upregulating PDE activity. ( A ) Forskolin-stimulated cAMP generated in uterine arteries from wild type (WT), Rgs2 knockout ( Rgs2 −/− ), Rgs5 knockout ( Rgs5 −/− ), and Rgs2/5 double knockout ( Rgs2/5 dbKO) mice. Freshly isolated uterine arteries were incubated with forskolin (50 μ m ) for 5 min in the presence of 50 μ m IBMX. ( B and C ) Phosphodiesterase (PDE) activity in uterine arteries from WT, Rgs2 −/− , Rgs5 −/− , and Rgs2/5 dbKO mice. Freshly isolated uterine arteries were incubated with vehicle (0.01% DMSO) or PTX (750 ng m −1 ) for 2 h before processing for the PDE activity assay. Data points indicate assays with uterine arteries from individual mice. Values are mean ± SEM. *, ** P < .05, .01 versus WT control (con); # P < .05, Rgs2 −/− con versus Rgs2 −/− + PTX; and ns—not significant.

    Article Snippet: Sample preparation and analysis were conducted in accordance with the protocol provided with the PDE Activity Assay Kit (No. ab241034, Abcam, MA).

    Techniques: Activity Assay, Generated, Knock-Out, Double Knockout, Isolation, Incubation